VAIBHAV ANITA SINGH May 17
Replying to @HPIndia @imVkohli
The champ z here... @imVkohli (Virat Kohli) #ReinventYourGame
SampleQuantityTechnical aspects and storageAnalyses
Peripheral venous blood10–20 mlPotassium oxalate and sodium fluoride as preservatives, stored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology
Peripheral venous blooda 10 mlEDTA, frozen optimally at − 80 °CGenetic analyses, Virology
Serum5–10 mlFrom peripheral venous blood centrifuged directly after sampling, stored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology, Chemistry
Vitreous humor5–10 mlStored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology, Chemistry
HairStrands (apx 0.2 g)From the vertex posterior, cut closely to the scalp, pulled out and containing hair bulbs if possible, stored in an aluminum foil, an envelope or in a plastic tube at ambient temperatureToxicology
Urine10–20 mlStored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology, Chemistry
BileAllStored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology
Pericardial/Cerebrospinal fluids5–10 mlStored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology, Chemistry
Gastric contentsWholeStored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology
Other tissue samplesb 25–50 gStored at 4° when analyzed promptly after autopsy, otherwise frozen at − 20°Toxicology
Heart/spleen5 gOptimally frozen at − 80 °C, otherwise
RNA later at room temperature; paraffin-embedded tissue are not optimal but might be considered
Genetic analyses, Virology
Blood or tissue culture5–10 gRecipients with specific media, depend on local laboratoryMicrobiology, Genetic analyses
Myocardium and other tissue samplesc 1 × 1 × 1 mmKarnovsky fixative, stored at 4°Ultrastructural analysis (electron microscopy)
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